FCRL5 / FITC / REA391

Product Details
Description Clone REA391 recognizes the human CD307e antigen, a 106 kDa single-pass type I membrane protein also known as Fc receptor-like protein 5 (FcRL5) or immune receptor translocation-associated protein 2 (IRTA2). CD307e possesses a cytoplasmic tail with inhibitory ITIM phosphorylation signaling motifs and was found to inhibit B cell antigen receptor signaling by recruiting SHP1 to two ITIM motifs upon B cell antigen receptor co-stimulation, resulting in diminished calcium influx and protein tyrosine phosphorylation. Co-stimulation of CD307e and the B cell antigen receptor promotes proliferation and differentiation of naive B cells. CD307e is expressed on both mature B cells and plasma cells, and is induced by EBV proteins. It is overexpressed on malignant B cells of hairy cell leukemia, chronic lymphocytic leukemia, mantle cell lymphoma, and multiple myeloma patients. In addition, serum levels of soluble CD307e are elevated in patients with several types of B cell tumors. | Additional information: Clone REA391 displays negligible binding to Fc receptors.
Conjugate FITC
Clone REA391
Target Species Human
Applications FC
Supplier Miltenyi Biotec
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About FCRL5
This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]
About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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