CD25 / Vio Bright R720 / REA568
Product Details
| Description | Clone REA568 recognizes the mouse CD25 antigen, the alpha chain of the IL-2 receptor (IL-2R). IL-2Ralpha associates with the beta chain (CD122) and the gamma chain (CD132) to form the functional IL-2R complex. IL-2Ralpha is expressed on CD4+CD25+ regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs). | Additional information: Clone REA568 displays negligible binding to Fc receptors. | | |
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| Conjugate | Vio Bright R720 | |
| Clone | REA568 | |
| Target Species | Mouse | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About CD25
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020]
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020]
About Vio Bright R720
Vio® Bright R720 and Vio® R720 have an excitation peak at 695 nm and an emission peak at 720 nm, which means that these dyes are optimally excited by the 640 nm red laser while the emitted light is collected by the channel with a 725/40 bandpass filter. Miltenyi Biotec's proprietary multimerization technology allows for Vio® Bright R720 to be nearly twice the brightness of Vio® R720, so this choice of fluorophore offers flexibility in your panel design in flow cytometry. Spectrally, Vio® Bright R720 is comparable to R700 from BD Biosciences.
Vio® Bright R720 and Vio® R720 have an excitation peak at 695 nm and an emission peak at 720 nm, which means that these dyes are optimally excited by the 640 nm red laser while the emitted light is collected by the channel with a 725/40 bandpass filter. Miltenyi Biotec's proprietary multimerization technology allows for Vio® Bright R720 to be nearly twice the brightness of Vio® R720, so this choice of fluorophore offers flexibility in your panel design in flow cytometry. Spectrally, Vio® Bright R720 is comparable to R700 from BD Biosciences.
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