About RPA2
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

About Alexa Fluor 594
Alexa Fluor™ 594 (AF594, Alexa 594) has an excitation peak at 590 nm and an emission peak at 617 nm, and is spectrally similar to Texas Red (ThermoFisher Scientific), DyLight™ 594 (ThermoFisher Scientific), iFluor® 594 (ATT Bioquest) and iFluor® 610 (ATT Bioquest), CF®594 (Biotium), and ATTO 594 (ATTO-TEC). Alexa 594 is commonly used for flow cytometry,fluorescence microscopy and super-resolution microscopy. It is very bright, photostable, and pH insensitive.