About CD86
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

About Brilliant Violet 510
Brilliant™ Violet 510 (BV510) is a green-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 510/50 bandpass filter. BV510 has an excitation peak at 405 nm and an emission peak at 510 nm, and is similar to StarBright Violet 515 (Bio-Rad). BV510 is most often used in flow cytometry due to its improved signal-to-noise over alternatives like V500 and AmCyan. This dye is part of the Brilliant™ Violet dye line. BV510 and BV480 are the only dyes in the Brilliant™ Violet dye family that are not tandem fluorophores based off the BV421 polymer core, but they have nearly the same maximum excitation.