About CD127
The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015]

About PE-Fire 640
PE-Fire™ 640 from Biolegend is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Fire™ 640. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Fire™ 640, which emitts light that can be captured with a 630/69 nm bandpass filter. PE-eF610 has an excitation peak at 565 nm and an emission peak at 639 nm. In spectral cytometry, this dye can be accurately unmixed from other dyes with highly overlapping spectral profiles, such as PE-Dazzle® 594 and PE-Cy5. PE-Fire™ 640 is recommended for labeling antigens that: (1) are expressed on a small subset (2) will be excluded from final analysis, and (3) require a bright signal but are not co-expressed with an antigen marked by a dye with overlapping spectra.