CD221 Monoclonal / PE / REA271
Product Details
| Description | Clone REA271 recognizes human CD221 (IGF-1R), a widely expressed cell-surface glycoprotein composed of two alpha subunits and two beta subunits. Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane alphabeta chain. The alpha chains are located extracellularly, while the beta subunits span the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF-1R is activated by binding of its ligands insulin-like growth factor 1 (IGF-1), IGF-2, as well as insulin to the extracellular alpha subunits. Ligand binding leads to autophosphorylation of tyrosine residues in the cytoplasmic regions of the receptor beta subunits and subsequent recruitment and phosphorylation of insulin receptor substrate (IRS) proteins. This finally results in activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells exhibit altered IGF-1R expression. | Additional information: Clone REA271 displays negligible binding to Fc receptors. | |
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| Conjugate | PE | |
| Clone | REA271 | |
| Target Species | Human | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
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About CD221
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
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Validation References
| PMID 18927274 | |
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