ADAM10 Monoclonal / FITC / REA309

Product Details

Description	Clone REA309 recognizes the human CD156c antigen, a single-pass type I membrane protein which is also known as disintegrin or metalloproteinase domain-containing protein 10 (ADAM10). CD156c belongs to the family of disintegrin and metalloproteinases (ADAMs) and is expressed ubiquitously on hematopoietic and non-hematopoietic cells. ADAMs have emerged as the major proteinase family that mediates proteolytic ectodomain release, a process known as shedding. This shedding process has been recognised as a key mechanism for regulating the function of a diversity of cell surface proteins. Dysregulation of ectodomain shedding is associated with autoimmune and cardiovascular diseases, neurodegeneration, infection, inflammation, and cancer. CD156c has been studied in the context of ectodomain shedding and has been demonstrated as key molecule in most of the shedding events in cancer and neurodegenerative disorders. \| Additional information: Clone REA309 displays negligible binding to Fc receptors. \|
Conjugate	FITC
Clone	REA309
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About ADAM10
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]

About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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