ERK1/2 (pT202/pY204) Monoclonal / PE / REA152

Product Details

Description	Clone REA152 recognizes the phosphorylated threonine 202 and tyrosine 204 (pT202/pY204) of human ERK1 and pT184/pY186 of human ERK2, which are key components of the MAPK signaling cascade. ERK1 and ERK2 are multifunctional serine/threonine kinases and are ubiquitously expressed in mammalian cells. They share 85% homology and are phosphorylated by kinases MEK1/2 at Thr and Tyr residues within their activation loop leading to activation and nuclear translocation of ERKs. Activated ERKs phosphorylate downstream targets, such as signaling effectors, receptors, cytoskeletal proteins, and transcription factors. Owing to such a wide array of cellular targets, ERKs are involved in regulation of a number of processes including cell adhesion, cell cycle progression, cell migration, cell survival, differentiation, metabolism, proliferation, and transcription. Activation and mobilization of ERKs is induced in response to several extracellular signals and engagement of receptors such as Tyr kinases (RTKs), G protein–coupled receptors (GPCRs), ion channels, and others. The ERK1/2 are dual specificity kinases and phosphorylate Ser or Thr residues neighbor to Proline residue. \| Additional information: Clone REA152 displays negligible binding to Fc receptors. \|
Conjugate	PE
Clone	REA152
Target Species	Human, Mouse
Applications	FC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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