Tetherin Monoclonal / FITC / REA202
Product Details
| Description | Clone REA202 recognizes the CD317 antigen, which is a lipid raft-associated type II transmembrane glycoprotein. CD317 is composed of a short N-terminal cytosolic domain, a transmembrane domain, and a C-terminal glycosyl-phosphatidylinositol anchor. Expression of CD317 is upregulated in the presence of IFN-alpha on T and B cells, is found on the apical surface of polarized epithelial cells, and on myeloma cells. CD317 expression is also found in an intracellular pool and the protein cycles between this intracellular pool and the cell surface in a clathrin dependent manner. It has been shown to play a role in regulating the growth and development of B cells, cell adhesion, cell migration, and in cultured cells. CD317 restricts the release of formed viral particles at the surface of infected cells. | Additional information: Clone REA202 displays negligible binding to Fc receptors. | | |
|---|---|---|
| Conjugate | FITC | |
| Clone | REA202 | |
| Target Species | Human | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
| Size | ||
| Price | ||
| Antigen | ||
| Host | ||
| Isotype |
About Tetherin
Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq, Jul 2008]
About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Experiment Design Tools
Panel Builders
Looking to design a Microscopy or Flow Cytometry experiment?
Validation References
| PMID 19273615 | |
|---|---|
| PMID 20147389 | |
| Additional Sources |
|
Reviews & Ratings
| Reviews |
|---|
Looking for more options?
399 Tetherin antibodies from over 25 suppliers available with over 67 conjugates.
