CD100 Monoclonal / FITC / REA316

Product Details

Description	Clone REA316 recognizes the human CD100 antigen, a single-pass type I membrane protein which is also known as semaphorin-4D (SEMA4D). CD100 is strongly expressed in skeletal muscle, and at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung, and pancreas. In the immune system, it is present on peripheral blood lymphocytes, spleen, and thymus, especially interfollicular T cells and B cells in the germinal centers of secondary lymphoid follicles. CD100 is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein and repels axon growth cones in the central nervous system. In the immune system, CD100 plays a critical role by turning off negative signaling by CD72 and significantly enhancing the effects of CD40 on B cell and dendritic cell responses. During skin damage repairs, SEMA4D interacts with plexin B2 on gamma delta T cells to play a role in the healing process. \| Additional information: Clone REA316 displays negligible binding to Fc receptors.
Conjugate	FITC
Clone	REA316
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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