NHE1 / FITC / 2F5

Product Details
Description SLC9A1 (Sodium/Hydrogen Exchanger 1, Na(+)/H(+) Exchanger 1, NHE-1, Solute Carrier Family 9 Member 1, Na(+)/H(+) Antiporter, Amiloride-sensitive, APNH, APNH1, NHE1, FLJ42224) (FITC) Mab 2F5
Conjugate FITC
Clone 2F5
Target Species Human
Applications ELISA, WB, IHC
Supplier US Biological
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About NHE1
This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]
About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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