CD55 Monoclonal / PE / REA300

Product Details

Description	Clone REA300 recognizes the CD55 antigen, a glycoprotein also known as complement decay-accelerating factor (DAF). There are two mouse CD55 genes, which share 85% nucleotide and 78% amino acid identities, and have been designated CD55-glycosylphosphatidylinositol and CD55-transmembrane to reflect the two alternate mechanisms of membrane attachment. Both proteins are recognized by clone REA300. CD55 is broadly distributed among hematopoietic and non-hematopoietic cells. It is expressed on the plasma membranes of all cell types that are in intimate contact with serum and it is also found on the surfaces of epithelial cells, lining extracellular compartments. CD55 plays multiple physiologic roles including tissue protection from the cytotoxic complement injury, anti-inflammatory function owing to its anti-adherence properties which enhance transmigration of monocytes and macrophages and reduce tissue injury. CD55 plays an essential role during pregnancy and is involved in the protection of the semiallogeneic fetus and in preventing uncontrolled infiltration by white cells. It is exploited in multiple pathologic conditions by pathogens and viruses in chronic tissue infection processes. \| Additional information: Clone REA300 displays negligible binding to Fc receptors. \|
Conjugate	PE
Clone	REA300
Target Species	Mouse
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About CD55
This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]

About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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