TGF-beta 1 Monoclonal / PE / CH6-17E5.1

Product Details
Description Clone CH6-17E5.1 reacts with the N-terminal latency-associated peptide (LAP) of the transforming growth factor beta1 (TGF-beta1) dimer. TGF-beta1 belongs to a family of homologous, disulfide-linked, homodimeric proteins. These highly pleiotropic cytokines inhibit proliferation of most cells, but can promote the growth of mesenchymal cells and enhance extracellular matrix formation. The pivotal function of TGF-beta1 in the immune system is to mediate immunosuppression and maintain tolerance by regulating lymphocyte proliferation, differentiation, and survival. TGF-beta1 is produced by many cell types, but is reported to be most abundant in mammalian platelets and bone. It is secreted predominantly as an inactive latent complex. After proteolytical processing of the TGF-beta1 precursor, the resulting N-terminal latency-associated peptide (LAP) remains non-covalently associated with the TGF-beta1 dimer. Mature and biologically active TGF-beta1 can be released from the complex by action of proteases and/or conformational changes. LAP-TGF-beta1 was recently described to be expressed on the cell surface of activated human regulatory T cells.
Conjugate PE
Clone CH6-17E5.1
Target Species Human
Applications FC
Supplier Miltenyi Biotec
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About TGF-beta 1
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]
About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
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Validation References
PMID 19299332
PMID 19651619
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