Siglec-5/Siglec-14 Monoclonal / PE / 1A5

Product Details

Description	Clone 1A5 recognizes the CD170 antigen, a single-pass transmembrane protein member of the immunoglobulin superfamily, also known as sialic acid-binding Ig-like lectin 5 (siglec-5). CD170 is expressed on neutrophils, monocytes, dendritic cells, and subsets of tissue macrophages. It contains two tyrosine-based motifs in its cytoplasmic tail implicating signaling functions and forms a 140 kDa dimer on the cell surface. Tyrosine phosphorylation of CD170 leads to recruitment of the tyrosine phosphatases SHP-1 and SHP-2, which in turn efficiently inhibits FcepsilonRI-mediated calcium fluxing and serotonin release. CD170 binds alpha 2-3– and alpha 2-6–linked sialic acid as well as glycophorin A, and is involved in cell adhesion.
Conjugate	PE
Clone	1A5
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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