Ly-49D Monoclonal / FITC / 4E5

Product Details
Description Clone 4E5 recognizes Ly-49D, a type II transmembrane NK-cell receptor belonging to the Ly-49 family. Members of Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands and include activating and inhibitory receptor molecules. Ly-49D is a prototypic activating member of Ly-49 family and in contrast to inhibitory members, lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). A positive signal is initiated by interaction of Ly-49D with immunoreceptor tyrosine-based activating motif (ITAM) containing transmembrane adapter DAP12. Expression of Ly-49D is strain restricted and is found on subsets of NK cells in C57BL/6, C3H/He, and SJL but not in BALB/c, DBA/2, AKR, and CBA/J mice.
Conjugate FITC
Clone 4E5
Target Species Mouse
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier Miltenyi Biotec
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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45 Ly-49D antibodies from over 3 suppliers available with over 28 conjugates.

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