About CD86
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

About Brilliant Blue 515
Horizon Brilliant™ Blue 515 (BB515), exclusive to BD Biosciences, is a brighter alternative to FITC and Alexa Fluor™ 488 in flow cytometry. It has an excitation peak at 590 nm and an emission peak at 515 nm.