About Live/Dead Fix Near IR (775)
The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Near IR (775) has an excitation peak at 750 nm and an emission peak at 775 nm. In flow cytometry, the 633 nm laser is typically used for excitation.