HLA BC / PE / REAL535

Product Details

Description	Clone REAL535 is an antibody fragment derived from the full HLA-BC antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity. \| Clone REAL535 recognizes the class I Human Leukocyte Antigens (HLA) B and C. Expressed on the surface of most nucleated cells, class I molecules are heterodimeric molecules and consist of a type I integral membrane alpha heavy chain and soluble beta2 microglobulin protein. The extracellular region of the heavy chain further consists of 3 domains, one of which comprises the peptide binding groove. Antigens binding to class I molecules are 8-10 amino acids long and play an important role in recognition of the virus infected and malignant cells by cytotoxic T lymphocytes (CTLs). In addition, class I molecules interact with NK cell receptors to modulate the activity of NK cells. \| For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).
Conjugate	PE
Clone	REAL535
Target Species	Human
Applications	MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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