Ly-49A/Ly-49D / PE / REAL400

Product Details

Description	Clone REAL400 is an antibody fragment derived from the full Anti-Ly-49A/D antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity. \| Clone REAL400 recognizes the mouse Ly-49A and Ly-49D antigens belonging to the Ly-49 family. Members of Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands, and include activating and inhibitory receptor molecules. Ly-49A is a 44 kDa type II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Expression of Ly-49A is found on subset of natural killer (NK) and NK-T cells. Ly-49D is a prototypic activating member of Ly-49 family and in contrast to inhibitory members, lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). A positive signal is initiated by interaction of Ly-49D with immunoreceptor tyrosine-based activating motif (ITAM) containing transmembrane adapter DAP12. Clone REAL400 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49. \| The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.
Conjugate	PE
Clone	REAL400
Target Species	Mouse
Applications	FC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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