Cytokeratin, HMW / FITC / REA914
Product Details
Description | Clone REA914 recognizes the human Cytokeratin HMW (High Molecular Weight), which includes the cytokeratins 1,5,10 and 14. Keratins are polypeptides with keratin-containing intermediate filaments, which play a critical role in mechanical stability and integrity of epithelial cells and tissues, have regulatory functions and are involved in intracellular signaling pathways. Clone REA914 detects squamous and ductal epithelium and other complex epithelia in normal tissues, e.g. basal cells in prostate, and myoepithelial cells in breast and corresponding tumors. | Additional information: Clone REA914 displays negligible binding to Fc receptors. | |
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Conjugate | FITC | |
Clone | REA914 | |
Target Species | Human | |
Applications | MICS (MACSima Imaging Cyclic Staining), IF, IHC | |
Supplier | Miltenyi Biotec | |
Catalog # | Sign in to view product details, citations, and spectra | |
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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Validation References
PMID 6186379 | |
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PMID 10521347 | |
PMID 9989840 | |
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