Cytokeratin 5/6 / FITC / REA913

Product Details

Description	Clone REA913 recognizes the human Cytokeratin 5/6, belonging to the basic or neutral type II cytokeratins. Cytokeratins, which are found in almost all epithelial cells, are polypeptides with keratin-containing intermediate filaments, which ensure form stability and cohesiveness. Cytokeratin 6 is located in proliferating squamous epithelium, whereas Cytokeratin 5 is found in the basal, the intermediate and the superficial cell layers of stratified and transitional epithelia, complex epithelia, and in mesothelial cells and mesothelioma, but not in simple epithelia and in non-epithelial cells. \| Additional information: Clone REA913 displays negligible binding to Fc receptors.
Conjugate	FITC
Clone	REA913
Target Species	Human
Applications	MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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