Ig Kappa Light Chain / FITC /

Product Details
Description Polyclonal Rabbit Anti-Human Kappa Light Chains/FITC, Rabbit F(ab')2, FITC, Flow Cytometry, 100 tests, 1 mL. This reagent has been produced in a manner that ensures a particularly wide specificity for kappa-chains. Most B cells, with the exception of pre-B progenitors, pre-B cells and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type. In normal peripheral blood and lymph nodes there is a mixture of kappa+ and lambda+ cells with two-thirds of the cells expressing kappa and one-third expressing lambda (1).;The reagents are of value for the demonstration of the monoclonal nature (light chain restriction) of lymphoid neoplasms.
Conjugate FITC
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Target Species Human
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Supplier DAKO
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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