CD66a/b/c/e / FITC /

Product Details

Description	Monoclonal Mouse Anti-Human CD66abce/FITC, Clone Kat4c, FITC, Flow Cytometry, 100 tests, 1 mL. CD66 refers to a family of heavily glycosylated glycoproteins whose members are designated CD66a to CD66f. CD66 antibodies often react with two or more members of this family, and antibody Kat4c recognizes three myeloid-associated molecules (CD66a, b, c) and also CD66e (CEA). In consequence, the antibody reacts with myeloid cells at differing stages of maturity (from promyelocytes to granulocytes), and also with a variety of epithelial cells. The antibody is of value in the immunophenotyping of leukemias of myeloid origin.
Conjugate	FITC
Clone
Target Species	Human
Applications
Supplier	DAKO
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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