Bcl-2 / DyLight 755 / 100/D5

Product Details

Description	This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Conjugate	DyLight 755
Clone	100/D5
Target Species	Human, Mouse (Negative), Rat (Negative)
Applications	ELISA, FC, ICC, IF, IHC-P, WB, IP, IHC-Fr, IHC
Supplier	Novus Biologicals
	Catalog #	Sign in to view product details, citations, and spectra
	Size
	Price
	Antigen
	Host
	Isotype

About Bcl-2
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

About DyLight 755
DyLight™ 755 has an excitation peak of 754 nm and an emission peak of 776 nm and is spectrally similar to Alexa Fluor™ 750. DyLight™ 755 is most commonly used in fluorescence micrscopy.

Experiment Design Tools