hapten 4-hydroxy-3-nitrophenyl acetyl (NP) Isotype Control / PerCP / S43.10

Product Details
Description The Mouse IgG2a isotype control antibody clone S43.10 is specific for the hapten NP (4-hydroxy-3-nitro-phenyl) acetyl. This hapten is not expressed on cells or cell lines. Therefore, the antibody clone S43.10 can be used as a negative control to distinguish specific from non-specific binding of mouse IgG2a fluorochrome-conjugated antibodies to human cells, for example, via Fc receptors or due to interactions of the fluorochrome with the cell surface.
Conjugate PerCP
Clone S43.10
Target Species Mouse
Applications FC
Supplier Miltenyi Biotec
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About PerCP
Peridinin-Chlorophyll-Protein (PerCP) is a red-emitting fluorescent protein isolated from algae that can be excited by the 488 nm blue laser and captured with a 670/30 nm bandpass filter. PerCP exhibits a large Stokes' Shift, with an excitation peak at 482 nm and an emission peak at 675 nm. PerCP is was historically used in flow cytometry, however it is highly susceptible to photobleaching and has poor stability. Alternatives like BB700, NovaFluor Blue 690 or PerCP-eFluorâ„¢ 710 are preferred. PerCP is a generic dye that has no sole manufacturer.
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Validation References
PMID 24098349
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138 hapten 4-hydroxy-3-nitrophenyl acetyl (NP) antibodies from over 3 suppliers available with over 36 conjugates.

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