CD52 / FITC / REA164

Product Details

Description	Clone REA164 recognizes CD52, a glycosylphosphatidylinositol (GPI)-anchored, 25–29 kDa heavily glycosylated antigen. The CD52 core peptide is only 12 amino acids long and is linked to the surface via C-terminal GPI anchor. Expression of CD52 is found on lymphocytes, monocytes, macrophages, eosinophils, and in the male reproductive tract on the epithelial cells of the epididymis and seminal vesicle. CD52 is absent in immature spermatozoa in the efferent ducts but is acquired by the sperm membrane, progressively, as they pass through the epididymis proper, where CD52 is shed into the seminal plasma by the epididymis. Though the exact role of CD52 is unclear, antibodies against CD52 elicit antibody dependent cellular and complement mediated cytolysis of the CD52 expressing lymphocytes. Owing to this, such antibodies are often used to target malignant cells. CD52 expressed on sperms and on lymphocytes are basically different glycoforms of the same core peptide. \| Additional information: Clone REA164 displays negligible binding to Fc receptors. \|
Conjugate	FITC
Clone	REA164
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About CD52
Involved in positive regulation of cytosolic calcium ion concentration. Predicted to be located in extracellular region and plasma membrane. Predicted to be intrinsic component of plasma membrane. Predicted to be active in sperm midpiece. [provided by Alliance of Genome Resources, Apr 2022]

About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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