About TCF7
This gene encodes a member of the T-cell factor/lymphoid enhancer-binding factor family of high mobility group (HMG) box transcriptional activators. This gene is expressed predominantly in T-cells and plays a critical role in natural killer cell and innate lymphoid cell development. The encoded protein forms a complex with beta-catenin and activates transcription through a Wnt/beta-catenin signaling pathway. Mice with a knockout of this gene are viable and fertile, but display a block in T-lymphocyte differentiation. Alternative splicing results in multiple transcript variants. Naturally-occurring isoforms lacking the N-terminal beta-catenin interaction domain may act as dominant negative regulators of Wnt signaling. [provided by RefSeq, Oct 2016]

About DyLight 633
DyLight™ 633 is a red-emitting fluroescent dye that can be excited by the 628 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 633 has an excitation peak of 638 nm and an emission peak of 658 nm, and is spectrally similar to Alexa Fluor™ 633. DyLight™ 633 is most commonly used in flow cytometery and fluorescence microscopy applications.