About nNOS
The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

About Alexa Fluor 680
Alexa Fluor™ 680 (AF680, Alexa 680) has an excitation peak at 679 nm and an emission peak at 702 nm, and is spectrally similar to Cy®5.5 (GE Healthcare), IR680® (Licor), iFluor® 680 (ATT Bioquest) and iFluor® 700 (ATT Bioquest). Alexa 680 is commonly used for flow cytometry, fluorescence microscopy and super-resolution microscopy.