About T-bet
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008]

About V450
V450 (Horizon V450) from BD Biosciences has an excitation peak at 406 nm and an emission peak at 450 nm. It is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), Vio®Blue® (Miltenyi Biotec), Alexa Fluor™ 405 (ThermoFisher Scientific), Cascade Blue® (ThermoFisher Scientific), Pacific Blue (ThermoFisher Scientific), and ATTO 425 (ATTO-Tec)