About CD86
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

About V450
V450 (Horizon V450) from BD Biosciences has an excitation peak at 406 nm and an emission peak at 450 nm. It is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), Vio®Blue® (Miltenyi Biotec), Alexa Fluor™ 405 (ThermoFisher Scientific), Cascade Blue® (ThermoFisher Scientific), Pacific Blue (ThermoFisher Scientific), and ATTO 425 (ATTO-Tec)