Dectin 1 / PE / REA515

Product Details

Description	Clone REA515 recognizes human DC-associated C-type lectin 1 (dectin-1), an approximately 33 kDa single-pass type II membrane protein, which is also known as C-type lectin domain family 7 member A (CLEC7A) or beta-glucan receptor (BGR). It promotes the phagocytosis of intact yeasts and glucan particles such as beta-1,3-linked and beta-1,6-glucans, thus playing an important role in anti-fungal immunity. In humans, eight isoforms of dectin-1 have been described. Only the two most common isoforms, dectin-1A and dectin-1B mediate the recognition of yeast particles in a beta-glucan-dependent manner. Monocytes express both dectin-1A and dectin-1B, while dectin-1A expression decreases during maturation to macrophages. Immature dendritic cells express high levels of both isoforms, but expression is lost when these cells are matured with LPS. Expression is also found on subpopulations of B cells, endothelial cells, eosinophils, fibroblasts, keratinocytes, Kupffer cells, Langerhans cells, mast cells, microglial cells, neutrophils, and T cells. In co-operation with TLR2 and TLR4, dectin-1 leads to cytokine production in human macrophages and monocytes. \| Additional information: Clone REA515 displays negligible binding to Fc receptors.
Conjugate	PE
Clone	REA515
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
	Catalog #	Sign in to view product details, citations, and spectra
	Size
	Price
	Antigen
	Host
	Isotype

About Dectin 1
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

Experiment Design Tools