Cytokeratin / FITC / CK3-6H5

Product Details

Description	Cytokeratins are typical intermediate filaments of the cytoskeleton of epithelial cells. They are a biochemically highly diverse multigene family of polypeptides with molecular masses ranging from 40 to 68 kDa. Until now 20 distinct cytokeratin polypeptides have been described, which can be divided into an acidic (type I) and a neutral-basic (type II) subfamily. The cytokeratin expression profile within one cell characterizes the type of epithelia and also the degree of maturation or differentiation. Most malignant cells which have their origin in epithelial tissue express a certain cytokeratin profile, which can be used for classifying carcinomas and for distinguishing carcinomas from malignant tumors of nonepithelial origin. The Anti-Cytokeratin (CK3-6H5) antibody is a pan-cytokeratin–specific antibody targeting cytokeratins from simple epithelia. It crossblocks Cam5.2, an antibody specific for cytokeratin 7 and 8, and has been shown to react with native cytokeratin preparations containing cytokeratins 8, 18, and 19 as well as with recombinant cytokeratin 8.
Conjugate	FITC
Clone	CK3-6H5
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

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