CD314 / PE-Vio615 / REA1175
Product Details
| Description | Clone REA1175 recognizes the mouse CD314 (NKG2D), a type II transmembrane homodimeric glycoprotein belonging to the C-type lectin-like receptor family. CD314 in mice is expressed on activated CD8+ alphabeta T cells, NK cells, subsets of gammadelta T cells, IFN-producing killer dendritic cells, subsets of NKT cells, and activated macrophages. For signaling, CD314 associates with signaling adapters DAP10 and DAP12 and transduces signal via the PI3-kinase pathway and the Syk-Zap70 pathways, respectively. CD314 recognizes multiple ligands, which are either uregulated in response to cellular stresses such as heat shock, infection, and DNA damage or are constitutively expressed, such as on tumor cells. Known ligands of CD314 in mice include murine UL16-binding protein-like transcript (MULTI), H60 family proteins and the five retinoic acid-inducible proteins RAE-1alpha, beta, gamma, delta, epsilon. Interaction of CD314 with its ligands regulates cytotoxic response towards the cells bearing the cognate ligands. | Additional information: Clone REA1175 displays negligible binding to Fc receptors. | |
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| Conjugate | PE-Vio615 | |
| Clone | REA1175 | |
| Target Species | Mouse | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About CD314
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster. [provided by RefSeq, Dec 2010]
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster. [provided by RefSeq, Dec 2010]
About PE-Vio615
PE-Vio®615 from Miltenyi Biotec has an excitation peak at 565 nm and an emission peak at 619 nm. It is spectrally similar to PE-eFluor™ 610, PE-Dazzle 594 and PE-CF®594
PE-Vio®615 from Miltenyi Biotec has an excitation peak at 565 nm and an emission peak at 619 nm. It is spectrally similar to PE-eFluor™ 610, PE-Dazzle 594 and PE-CF®594
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Validation References
| PMID 11248803 | |
|---|---|
| PMID 12150888 | |
| PMID 12426564 | |
| PMID 18209064 | |
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