CD276 / PE / FM276

Product Details
Description Clone FM276 reacts with human CD276 (B7-H3), a type I transmembrane protein expressed at low levels in human liver, lung, bladder, testis, prostate, breast, placenta, lymphoid organs, and many human cancers. Surface expression can be induced on T cells, natural killer cells, and macrophages as well as on dendritic cells (DC) by IFN-gamma and on monocytes by granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). B7-H3 has been shown to function as costimulatory molecule for T cell activation by inducing T cell proliferation and IFN-gamma production.
Conjugate PE
Clone FM276
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier Miltenyi Biotec
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About CD276
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
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569 CD276 antibodies from over 29 suppliers available with over 69 conjugates.

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