TNF alpha / PE / cA2
Product Details
| Description | Tumor necrosis factor alpha (TNF-alpha) is produced by cells that are involved in inflammatory immune responses. TNF-alpha is secreted by activated CD4+ T cells, monocytes, macrophages, NK cells, and neutrophils. | Anti-TNF-alpha antibodies have been designed for intracellular staining of TNF-alpha–producing cells. Cells can be stimulated for TNF-alpha production, for example, by polyclonal stimulation with mitogens. For induction of TNF-alpha production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-alpha can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-alpha–producing cells can be stained intracellularly with Anti-TNF-alpha antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-alpha–producing cells. | |
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| Conjugate | PE | |
| Clone | cA2 | |
| Target Species | Human | |
| Applications | FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
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