TNF alpha / APC-Vio770 / cA2

Product Details

Description	Tumor necrosis factor alpha (TNF-alpha) is produced by cells that are involved in inflammatory immune responses. TNF-alpha is secreted by activated CD4+ T cells, monocytes, macrophages, NK cells, and neutrophils. \| Anti-TNF-alpha antibodies have been designed for intracellular staining of TNF-alpha–producing cells. Cells can be stimulated for TNF-alpha production, for example, by polyclonal stimulation with mitogens. For induction of TNF-alpha production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-alpha can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-alpha–producing cells can be stained intracellularly with Anti-TNF-alpha antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-alpha–producing cells.
Conjugate	APC-Vio770
Clone	cA2
Target Species	Human
Applications	FC
Supplier	Miltenyi Biotec
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About APC-Vio770
APC-Vio®770 from Miltenyi is a tandem fluorophore with APC as the donor and has an excitation peak at 652 nm and an emission peak at 775 nm, and is spectrally similar to APC-Cy® 7 (GE Healthcare) APC-H7 (BD Biosciences), APC-eFluor™ 780 (ThermoFisher Scientific), APC-Vio®770 (Miltenyi Biotec), and APC-Fire™ 750 (BioLegend). The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.

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