TNF alpha / APC / cA2

Product Details

Description	Tumor necrosis factor alpha (TNF-alpha) is produced by cells that are involved in inflammatory immune responses. TNF-alpha is secreted by activated CD4+ T cells, monocytes, macrophages, NK cells, and neutrophils. \| Anti-TNF-alpha antibodies have been designed for intracellular staining of TNF-alpha–producing cells. Cells can be stimulated for TNF-alpha production, for example, by polyclonal stimulation with mitogens. For induction of TNF-alpha production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-alpha can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-alpha–producing cells can be stained intracellularly with Anti-TNF-alpha antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-alpha–producing cells.
Conjugate	APC
Clone	cA2
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About APC
Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.

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