TIM-4 / FITC / REA999
Product Details
| Description | Clone REA999 recognizes the mouse antigen TIM-4, which is also known as T cell immunoglobulin and mucin domain-containing protein 4 or spleen, mucin-containing, knockout of lymphotoxin protein (SMUCKLER). TIM-4 is a member of the TIM gene family, which is involved in a variety of immunity related processes including T cell proliferation and survival, tissue inflammation, and atopy. In mice, eight TIM genes encode the proteins TIM-1 to TIM-8, whereas only three TIM genes are found in humans encoding TIM-1, TIM-3, and TIM-4. In spleen, TIM-4 is expressed on antigen presenting cells such as lymphoid and myeloid dendritic cells and macrophages but not by T or B lymphocytes. | |
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| Conjugate | FITC | |
| Clone | REA999 | |
| Target Species | Mouse | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About TIM-4
Predicted to enable phosphatidylserine binding activity. Predicted to act upstream of or within apoptotic cell clearance. Predicted to be integral component of membrane. [provided by Alliance of Genome Resources, Apr 2022]
Predicted to enable phosphatidylserine binding activity. Predicted to act upstream of or within apoptotic cell clearance. Predicted to be integral component of membrane. [provided by Alliance of Genome Resources, Apr 2022]
About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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Validation References
| PMID 14768054 | |
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| PMID 17960135 | |
| PMID 18354194 | |
| Additional Sources |
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