Nanog / PE / REA314

Product Details

Description	Clone REA314 recognizes the human Nanog antigen, a homeobox transcription factor also known as ES cell–associated protein 4. Nanog is thought to be a key factor in maintaining pluripotency. It is expressed in the central portion of the morula, the inner cell mass of the blastocyst, in embryonic stem cells (ESC), and embryonic germ cells (EGC). The expression decreases with ESC differentiation. Nanog is thought to function in concert with other factors such as Oct-4 and SOX2 to establish ESC identity. Inhibition of Nanog expression has led to a reduction in tumorigenicity and such in vitro characteristics of stem cells as anchorage independent growth whereas overexpression may increase tumorigenicity. \| Additional information: Clone REA314 displays negligible binding to Fc receptors.
Conjugate	PE
Clone	REA314
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About Nanog
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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