IFN-gamma / PE-Vio770 / 45-15
Product Details
| Description | Interferon gamma (IFN-gamma) is produced by cells that are involved in inflammatory immune responses. IFN-gamma is predominantly released by memory and effector CD4+ and CD8+ T cells as well as by NK cells upon activation. Quantitative analysis of IFN-gamma–producing cells can provide important information on the course of immune responses. Anti-IFN‑gamma antibodies are designed for intracellular staining of IFN‑gamma–producing cells. Cells can be stimulated for IFN-gamma–production, e.g. by polyclonal stimulation with mitogens. For induction of IFN‑gamma–production by antigen-specific T cells, cells are restimulated with respective antigen. IFN‑gamma can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IFN‑gamma–producing cells can be stained intracellularly with Anti-IFN‑gamma antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IFN-gamma–producing cells. | |
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| Conjugate | PE-Vio770 | |
| Clone | 45-15 | |
| Target Species | Human | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
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About IFN-gamma
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
About PE-Vio770
PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
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Validation References
| PMID 23525357 | |
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