6xHis Tag / FITC / GG11-8F3.5.1
Product Details
| Description | The monoclonal Anti-His antibody facilitates the fast and convenient detection of His-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The conjugated antibody allows fast and convenient analysis of His-tagged fusion proteins. Conjugation of the Anti-His antibody to HRP simplifies Western blot or ELISA analysis as the incubation with secondary antibodies becomes dispensable. Fluorochorme-conjugated Anti-His antibodies enable direct flow cytometry and fluorescent microscopy analyses, while indirect fluorescent labeling of His fusion protein expressing cells is possible with the biotin-conjugated antibody. After incubation the Anti-His-HRP antibody can be directly detected using commercially available chemiluminescent reagents. | The monoclonal Anti-His antibody specifically recognizes proteins that contain a polyhistidine tag at their N- and C-terminus. However, the Anti-His-HRP (C-term.) antibody shows higher sensitivity and specificity for C-terminal His-tagged proteins. | |
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| Conjugate | FITC | |
| Clone | GG11-8F3.5.1 | |
| Target Species | All Species | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About FITC
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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