CD64 / PE / REA286

Product Details

Description	Clone REA286 recognizes the CD64 antigen, a 72 kDa integral membrane glycoprotein also known as high affinity immunoglobulin gamma Fc receptor I (FcgammaRI). Structurally CD64 is composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively expressed on macrophages, monocytes, dendritic cells, and mast cells. Treatment with cytokines like IFN-gamma and G-CSF induces CD64 upregulation. CD64 binds monomeric IgG-type antibodies with high affinity and mediates endocytosis, phagocytosis, antibody-dependent cellular toxicity, cytokine release, and superoxide generation. \| Additional information: Clone REA286 displays negligible binding to Fc receptors. \|
Conjugate	PE
Clone	REA286
Target Species	Mouse
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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