CD62P / PE / REA389

Product Details

Description	Clone REA389 recognizes the human CD62P antigen, a 140 kDa single-pass type I membrane protein also known as P-selectin or platelet activation dependent granule-external membrane protein (PADGEM). CD62P is expressed by vascular endothelial cells and platelets. The protein is stored preformed in the Weibel-Palade bodies of endothelial cells and in the alpha-granules of platelets. Upon stimulation of these cells with a variety of agonists such as thrombin or histamine, CD62P is rapidly translocated to the cell surface, where it mediates leukocyte-platelet and leukocyte-vascular endothelial cell adhesion. The interaction of CD62P with mature leukocytes is in part responsible for mediating leukocyte rolling on vascular cells – an early event in leukocyte recruitment at sites of inflammation. In addition to its role as a receptor for mature neutrophils and monocytes, CD62P also functions as a cell adhesion molecule for leukocyte precursor cells in the bone marrow, including both lineage-restricted clonogenic progenitors and hierarchically more primitive progenitor cells. \| Additional information: Clone REA389 displays negligible binding to Fc receptors.
Conjugate	PE
Clone	REA389
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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