CD3 / PerCP-Vio700 / REA641
Product Details
| Description | Clone REA641 recognizes the mouse CD3 complex, which is expressed on all mature T lymphocytes, NKT cells, and during the development of thymocytes. CD3, also known as T3, belongs to the Ig superfamily. It consists of CD3epsilon, delta, gamma, and zeta chains and forms a TCR complex by associating with TCR alpha/beta or gamma/delta chains. The TCR does not possess intracellular signaling domains, uncoupling Ag recognition from T cell signaling. The TCR is instead non-covalently associated with a multisubunit signaling apparatus, consisting of the CD3epsilongamma and CD3epsilondelta heterodimers and the CD3zetazeta homodimer, which collectively form the TCR-CD3 complex. CD3 is involved in T cell activation, signal transduction, and antigen recognition by binding the peptide/MHC antigen complex. | Additional information: Clone REA641 displays negligible binding to Fc receptors. | |
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| Conjugate | PerCP-Vio700 | |
| Clone | REA641 | |
| Target Species | Mouse | |
| Applications | FC | |
| Supplier | Miltenyi Biotec | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About PerCP-Vio700
PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
PerCP-Vio® 770 is a far-red-emitting tandem fluorophore that combines PerCP and a Vio®770 dye. The donor, PerCP, can be excited by the 488 nm blue laser and transfers energy to the acceptor, Vio®770, which emits light that can be captured with a 780/60 nm bandpass filter. APC-Vio®770 has an excitation peak at 482 nm and an emission peak at 704 nm, and is spectrally similar to PerCP-Cy5.5 and PerCP-eF710. PerCP-Vio®770 is known to be very bright and photostable, as well as being stable when treated with fixatives. These favorable characteristics that make it useful in multiple different applications such as flow cytometry and Fluorescence Microscopy. The large stokes shift can be advantageous when trying to fit more colors into a multicolor panel. The Vio® dye family are products of Miltenyi Biotec, with many antibody conjugates designed and validated for flow cytometry.
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Validation References
| PMID 1686832 | |
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| PMID 2534389 | |
| PMID 3478717 | |
| PMID 25422432 | |
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