Siglec-5/Siglec-14 / PE / REA1077

Product Details

Description	Clone REA1077 recognizes the CD170 antigen, a single-pass transmembrane protein member of the immunoglobulin superfamily, also known as sialic acid-binding Ig-like lectin 5 (Siglec-5). CD170 is expressed on neutrophils, monocytes, dendritic cells, and subsets of tissue macrophages. It contains two tyrosine-based motifs in its cytoplasmic tail implicating signaling functions and forms a 140 kDa dimer on the cell surface. Tyrosine phosphorylation of CD170 leads to recruitment of the tyrosine phosphatases SHP-1 and SHP-2, which in turn efficiently inhibits FcepsilonRI-mediated calcium fluxing and serotonin release. CD170 binds alpha 2-3- and alpha 2-6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion. \| Additional information: Clone REA1077 displays negligible binding to Fc receptors.
Conjugate	PE
Clone	REA1077
Target Species	Human
Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Supplier	Miltenyi Biotec
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About PE
Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.

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