Rab4 / ATTO 594 /

Product Details
Description Rabbit polyclonal to Rab4 (ATTO 594). Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment. Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. Each Rab shows a characteristic sub-cellular distribution. In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol....
Conjugate ATTO 594
Clone
Target Species Human, Mouse, Rat
Applications IF, ICC, WB
Supplier Biorbyt
Catalog # Sign in to view product details, citations, and spectra
Size
Price
Antigen
Host
Isotype
About ATTO 594
ATTO 594 from ATTO-Tec Gmbh has an excitation peak at 601 nm and an emission peak at 627 nm.
Experiment Design Tools
Panel Builders

Looking to design a Microscopy or Flow Cytometry experiment?

Validation References
Additional
Sources
Reviews & Ratings
Looking for more options?

103 Rab4 antibodies from over 14 suppliers available with over 33 conjugates.

Supplier Page
 Compare