About Alexa Fluor 647

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Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

Alternative Dyes to Alexa Fluor 647

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ATTO 647 ATTO 647N Cy5

Dyes similar to Alexa Fluor 647 based on emission

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APC ATTO 633 ATTO 647 ATTO 647N Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 CF640R CF647 CF647ST CF650 Calcein Deep Red AM CellBrite Fix 640 CellBrite Red CellROX Deep Red CellTrace Far Red CellVue Maroon CellVue Plum Chromeo 642 CoraLite Plus 647 Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DY-481XL DY-520XL DY-521XL DiD DyLight 633 DyLight 649 DyLight 650 Dyomics 647 ExoBrite 640/660 EV Membrane Stain FLUX 640 FLUX 647 FVS660 Flash Phalloidin NIR 647 FluoProbes647H Fura Red AM (Ca2+bound) Fura Red AM (Ca2+free) FxCycle Far Red IRDye 650 Janelia Fluor 646 LipidSpot 610 Live-or-Dye 640/662 Live/Dead Fix Far Red LysoView 633 LysoView 640 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MitoView 650 NL 637 NeuroVue Maroon Nile Blue NirFP NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 NucSpot Far-Red Oyster 650 SureLight P3 PE-Alexa 647 PE-Cy5 PE-Dyomics 647 PhenoVue Fluor 647 PhenoVue Fluor 647 Live Cell PhenoVue ROS-670, Total Oxidative Stress Indicator PhenoVue 641 Mitochondrial Stain PromoFluor 633P PromoFluor 647P ROS-ID Red RPE-CF647T ReadiLink 647-674 STAR 635 STAR RED Seta-650-NHS Seta-R-PE-670 StarBright UltraViolet 665 StarBright Violet 670 StarBright Yellow 665 SureLight APC SureLight PE Cyanine 5.0 TO-Pro 3 TOTO-3 TSA Vivid 650 Thiazole Red Thiazole Red Homodimer Tri-Color Vio667 VioBright 667 VivaFix 649/660 eFluor 660 eFluor 660 Fix Viability eFluor 670 Cell Proliferation iFluor 647 iRFP 670