Brilliant Ultraviolet 496 Dye Profile

BD Biosciences™

355

Laser Line

515/30

Common Filter

348

Excitation Max

496

Emission Max

About Brilliant Ultraviolet 496


Brilliant™ UltraViolet 496 (BUV496) is a blue-emitting tandem fluorophore that combines BD Horizon® BUV395 and an acceptor dye. It can be excited by the 355 nm ultraViolet laser and collected using a 515/30 bandpass filter. BUV496 has an excitation peak at 348 nm and an emission peak at 496 nm BUV496 has a low/medium relative brightness, and is most commonly used for multicolor flow cytometry. This dye is part of the Brilliant™ UltraViolet dye family, developed and sold by BD Biosciences. This family of dyes uses BUV395 as the donor so that all dyes in the family can be excited by the 355 nm ultraViolet laser. Depending on the acceptor, the dyes in this family have different relative brightness ratings.



Spectra


Alternative Dyes to Brilliant Ultraviolet 496

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StarBright UltraViolet 510


Popular Brilliant Ultraviolet 496 Antibodies


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