About Live/Dead Fix Blue

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The Live/Dead Fixable Viability probes from Thermo Fisher are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live/Dead Fixable Blue has an excitation peak at 341 nm and an emission peak at 440 nm.

Alternative Dyes to Live/Dead Fix Blue

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Ghost Dye UV 450 VivaFix 353/442 FVS440UV

Dyes similar to Live/Dead Fix Blue based on emission

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4-MUP 7-Amino-4-methylcoumarin (AMC) AMCA Alexa Fluor 350 BFP CF350 CF405S Calcein Blue Calcein Blue AM Calcein Violet AM CellBrite Blue CellBrite Blue Atlantic Blue CoraLite Plus 405 CytoTrack Blue DiFMU pH3.0 DiFMU pH9.0 DyLight 350 FVS440UV FVS450 Ghost Dye UV 450 Ghost Dye Violet 450 Hoechst (34580) LysoSensor Yellow/Blue pH9.0 MemBrite Fix 405/430 MitoView 405 PromoFluor 350 StarBright UltraViolet 445 StarBright Violet 440 SuperBright 436 V450 VivaFix 353/442 Vybrant DyeCycle Violet eBFP eFluor 450 Cell Proliferation eFluor 450 Fix Viability