About Spark Red 718

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Spark Red™ 718 is a brighter alternative to Alexa Fluor® 700, adding to the fluorophore options for the red laser that can detect antigens with lower expression levels. It can be used on a conventional cytometer with a 633 nm red laser and 720/45 filter set (or equivalent filter used to detect Alexa Fluor® 700) spectral cytometers with appropriate instrument settings. On conventional cytometers, Spark Red™ 718 can be utilized with both APC and APC/Cyanine7. On spectral cytometers, it can be unmixed from APC, Spark NIR™ 685, Alexa Fluor® 660, APC/Cyanine7, and APC/Fire™ 810. As spectral properties of Spark Red™ 718 and Alexa Fluor® 700 are nearly identical, using them together in a multicolor panel is not recommended. With a medium brightness, Spark Red™ 718 is best suited for detecting antigens with low to moderate expression levels.

Dyes similar to Spark Red 718 based on emission

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APC-Alexa 680 APC-Alexa 700 APC-R700 ATTO 700 Alexa Fluor 680 Brilliant Violet 711 CF680R CellBrite Steady 685 CellTrace CFSE CellVue Burgundy CoraLite Plus 750 DyLight 680 Ghost Dye Red 710 IFP 1.4 LDS 751 LysoView 680 MemBrite Fix 680/700 NeuroVue Burgundy NovaFluor Red 710 NovaFluor Yellow 730 NucSpot 680 PE-Alexa 700 PerCP-Vio700 PerCP-eFluor 710 PromoFluor 680 Qdot 705 ReadiLink 680-701 ReadiLink 700-713 Red 718 SeTau-665-NHS SeTau-680-NHS Seta-APC-705 StarBright Violet 710 StarBright Yellow 720 SuperBright 702 SureLight PerCP Cyanine 5.5 iFluor 680 iFluor 700 iRFP iRFP 702 redFluor 710